كتاب (Capillary Electrophoresis of Nucleic Acids Volume II Practical Appالكتب العلمية

كتاب (Capillary Electrophoresis of Nucleic Acids Volume II Practical App

نبذه عن الكتاب: 1. Introduction Since the first descriptions of electrophoresis in small diameter tubes in the 1970s and 1980s (1,2), capillary electrophoresis (CE) has been recognized for its potential to replace slab-gel electrophoresis for the analysis of nucleic acids (3,4). In particular, the availability of commercial instrumentation for CE over the last several years has made both the size determination and quantitation of DNA restriction fragments or polymerase chain reaction (PCR) products amenable to automation. Due to the same charge-to-mass ratio, the electrophoretic mobility of nucleic acid molecules in free solution is largely independent of their molecular size (5). Therefore, a sieving medium is required for the electrophoretic analysis of DNA fragments based on their size. Typically, two different principal types of separation matrix are used. The first type of matrix is of high viscosity polymer (e.g., polyacrylamide) with a well-defined crosslinked gel in regard to the structure and size of its pores. The second type of matrix is a noncrosslinked linear polymer network of materials such as, linear polyacrylamide, agarose, cellulose, dextran, poly(ethylene oxide), with lower viscosity than the former type and with a more dynamic pore structure. Although the first type of matrix is attached covalently to the capillary wall and may provide better separation for small (sequencing) fragments, the second matrix format has the advantage of being able to be replenished after each electrophoretic cycle. This typically extends the lifetime of a capillary, prevents contamination of the system, avoids sample carryover and allows the use of temperatures well above room temperature. Most matrices used in both systems are tolerant to the addition of DNA denaturants. Many different media useful for the separation of DNA have now become commercially available (6). In summary, the application of CE for DNA related research is attractive for numerous reasons: 1. The high degree of automation avoids cumbersome gel pouring and sample loading. 2. High mass sensitivity eliminates the need to label DNA with carcinogenic stains, or with radioactive DNA precursors
Keith R. Mitchelson Jing Cheng - ❰ له مجموعة من الإنجازات والمؤلفات أبرزها ❞ (Capillary Electrophoresis of Nucleic Acids Volume II Practical App ❝ ❱
من Biology Books علم الأحياء - مكتبة الكتب العلمية.

وصف الكتاب : نبذه عن الكتاب:

1. Introduction
Since the first descriptions of electrophoresis in small diameter tubes in the 1970s and
1980s (1,2), capillary electrophoresis (CE) has been recognized for its potential to replace
slab-gel electrophoresis for the analysis of nucleic acids (3,4). In particular, the availability
of commercial instrumentation for CE over the last several years has made both the size
determination and quantitation of DNA restriction fragments or polymerase chain reaction
(PCR) products amenable to automation. Due to the same charge-to-mass ratio, the electrophoretic mobility of nucleic acid molecules in free solution is largely independent of
their molecular size (5). Therefore, a sieving medium is required for the electrophoretic
analysis of DNA fragments based on their size. Typically, two different principal types of
separation matrix are used. The first type of matrix is of high viscosity polymer (e.g.,
polyacrylamide) with a well-defined crosslinked gel in regard to the structure and size of
its pores. The second type of matrix is a noncrosslinked linear polymer network of materials such as, linear polyacrylamide, agarose, cellulose, dextran, poly(ethylene oxide), with
lower viscosity than the former type and with a more dynamic pore structure. Although the
first type of matrix is attached covalently to the capillary wall and may provide better
separation for small (sequencing) fragments, the second matrix format has the advantage
of being able to be replenished after each electrophoretic cycle. This typically extends the
lifetime of a capillary, prevents contamination of the system, avoids sample carryover and
allows the use of temperatures well above room temperature. Most matrices used in both
systems are tolerant to the addition of DNA denaturants. Many different media useful for
the separation of DNA have now become commercially available (6).
In summary, the application of CE for DNA related research is attractive for
numerous reasons:
1. The high degree of automation avoids cumbersome gel pouring and sample loading.
2. High mass sensitivity eliminates the need to label DNA with carcinogenic stains, or with
radioactive DNA precursors

عدد مرات التحميل : 3463 مرّة / مرات.
تم اضافته في : الإثنين , 20 يناير 2020م.
حجم الكتاب عند التحميل : 3.037 .

ولتسجيل ملاحظاتك ورأيك حول الكتاب يمكنك المشاركه في التعليقات من هنا:

Biologically

Biology is a natural science that is concerned with the study of life, its various forms and its function, how these organisms interact with each other and with the surrounding environment. The word biology in Greek is made up of two words: bio (βίος) meaning life. And loggia (-λογία) means science or study. Biology: the similarity of vegetation and animal cover on the edges of the African and American states, and the existence of the same fossil.


Branches of biology
Biology is an ancient science thousands of years old and modern biology began in the nineteenth century. This science has multiple branches. Among them are:

Anatomy
Botany
Biochemia
Biogeography
Biofisia
Cytology or cell science
Ecology or environmental science

 

 

نبذه عن الكتاب:

1. Introduction
Since the first descriptions of electrophoresis in small diameter tubes in the 1970s and
1980s (1,2), capillary electrophoresis (CE) has been recognized for its potential to replace
slab-gel electrophoresis for the analysis of nucleic acids (3,4). In particular, the availability
of commercial instrumentation for CE over the last several years has made both the size
determination and quantitation of DNA restriction fragments or polymerase chain reaction
(PCR) products amenable to automation. Due to the same charge-to-mass ratio, the electrophoretic mobility of nucleic acid molecules in free solution is largely independent of
their molecular size (5). Therefore, a sieving medium is required for the electrophoretic
analysis of DNA fragments based on their size. Typically, two different principal types of
separation matrix are used. The first type of matrix is of high viscosity polymer (e.g.,
polyacrylamide) with a well-defined crosslinked gel in regard to the structure and size of
its pores. The second type of matrix is a noncrosslinked linear polymer network of materials such as, linear polyacrylamide, agarose, cellulose, dextran, poly(ethylene oxide), with
lower viscosity than the former type and with a more dynamic pore structure. Although the
first type of matrix is attached covalently to the capillary wall and may provide better
separation for small (sequencing) fragments, the second matrix format has the advantage
of being able to be replenished after each electrophoretic cycle. This typically extends the
lifetime of a capillary, prevents contamination of the system, avoids sample carryover and
allows the use of temperatures well above room temperature. Most matrices used in both
systems are tolerant to the addition of DNA denaturants. Many different media useful for
the separation of DNA have now become commercially available (6).
In summary, the application of CE for DNA related research is attractive for
numerous reasons:
1. The high degree of automation avoids cumbersome gel pouring and sample loading.
2. High mass sensitivity eliminates the need to label DNA with carcinogenic stains, or with
radioactive DNA precursors

Biology
Human biology
Who is the founder of biology?
The importance of biology
Areas of work in the field of biology
Theories of biology
Research on biology for the first grade of secondary school
Human biology

 



نوع الكتاب : pdf.
اذا اعجبك الكتاب فضلاً اضغط على أعجبني
و يمكنك تحميله من هنا:

تحميل (Capillary Electrophoresis of Nucleic Acids Volume II Practical App
Keith R. Mitchelson
Jing Cheng
Keith R. Mitchelson Jing Cheng



كتب اخرى في Biology Books

(An introduction to continuous-time stochastic processes theory, models, and applications to finance PDF

قراءة و تحميل كتاب (An introduction to continuous-time stochastic processes theory, models, and applications to finance PDF مجانا

(Artificial Intelligence and Molecular Biology-AAAI Press MIT Press [distributor] (1993 PDF

قراءة و تحميل كتاب (Artificial Intelligence and Molecular Biology-AAAI Press MIT Press [distributor] (1993 PDF مجانا

(Branching Processes in Biology-Springer New York (2002 PDF

قراءة و تحميل كتاب (Branching Processes in Biology-Springer New York (2002 PDF مجانا

Mathematical models in biology solution manual-Cambridge University Press (2003) PDF

قراءة و تحميل كتاب Mathematical models in biology solution manual-Cambridge University Press (2003) PDF مجانا

Chromatin Protocols-Humana Press PDF

قراءة و تحميل كتاب Chromatin Protocols-Humana Press PDF مجانا

Mathematical models in biology. An introduction-Cambridge University Press PDF

قراءة و تحميل كتاب Mathematical models in biology. An introduction-Cambridge University Press PDF مجانا

Hunter.-Molecular biology for computer scientists PDF

قراءة و تحميل كتاب Hunter.-Molecular biology for computer scientists PDF مجانا

Hiroaki Kitano-Foundations of Systems Biology-MIT Press PDF

قراءة و تحميل كتاب Hiroaki Kitano-Foundations of Systems Biology-MIT Press PDF مجانا

المزيد من المعاجم والقواميس في اللغة العربية في مكتبة المعاجم والقواميس في اللغة العربية , المزيد من النحو في مكتبة النحو , المزيد من كتب علمية في مكتبة كتب علمية , المزيد من كتب الجغرافيا والرحلات في مكتبة كتب الجغرافيا والرحلات , المزيد من كتب علم الفيزياء في مكتبة كتب علم الفيزياء , المزيد من علم نفس واجتماع في مكتبة علم نفس واجتماع , المزيد من رسائل ماجستير و دكتوراه فى الارشاد و علم النفس في مكتبة رسائل ماجستير و دكتوراه فى الارشاد و علم النفس , المزيد من كتب علم الرياضيات في مكتبة كتب علم الرياضيات , المزيد من كتب علم الزراعة في مكتبة كتب علم الزراعة
عرض كل الكتب العلمية ..
اقرأ المزيد في مكتبة كتب إسلامية , اقرأ المزيد في مكتبة كتب تقنية المعلومات , اقرأ المزيد في مكتبة المناهج التعليمية والكتب الدراسية , اقرأ المزيد في مكتبة القصص والروايات والمجلّات , اقرأ المزيد في مكتبة كتب الهندسة والتكنولوجيا , اقرأ المزيد في مكتبة الكتب والموسوعات العامة , اقرأ المزيد في مكتبة كتب تعلم اللغات , اقرأ المزيد في مكتبة كتب التنمية البشرية , اقرأ المزيد في مكتبة الكتب التعليمية , اقرأ المزيد في مكتبة كتب التاريخ , اقرأ المزيد في مكتبة كتب الأطفال قصص ومجلات , اقرأ المزيد في مكتبة كتب الطب , اقرأ المزيد في مكتبة الكتب العلمية , اقرأ المزيد في مكتبة كتب علوم سياسية وقانونية , اقرأ المزيد في مكتبة كتب الأدب , اقرأ المزيد في مكتبة كتب الروايات الأجنبية والعالمية , اقرأ المزيد في مكتبة كتب اللياقة البدنية والصحة العامة , اقرأ المزيد في مكتبة كتب الأسرة والتربية الطبخ والديكور , اقرأ المزيد في مكتبة الكتب الغير مصنّفة , اقرأ المزيد في مكتبة كتب المعاجم واللغات , اقرأ المزيد في مكتبة كتب علوم عسكرية و قانون دولي
جميع مكتبات الكتب ..