كتاب Methods in Molecular Biology Expression Protocolsالكتب العلمية

كتاب Methods in Molecular Biology Expression Protocols

نبذه عن الكتاب: The aim of E. coli Gene Expression Protocols is to familiarize and instruct the reader with currently popular and newly emerging methodologies that exploit the advantages of using E. coli as a host organism for expressing recombinant proteins. The chapters generally fall within two categories: (1) the use of E. coli vectors and strains for production of pure, functional protein, and (2) the use of E. coli as host for the functional screening of large collections of proteins or peptides. These methods and protocols should be of use to researchers over a wide range of disciplines. Chapters that fall within the latter category describe protocols that will be particularly relevant for functional genomics studies. The chapters of E. coli Gene Expression Protocols are written by experts who have hands-on experience with the particular method. Each article is written in sufficient detail so that researchers familiar with basic molecular techniques and experienced with handling E. coli and its bacteriophages should be able to carry out the procedures successfully. As in all volumes of the Methods in Molecular Biology series, each chapter includes an extensive Notes section, in which practical details peculiar to the particular method are described. E. coli Gene Expression Protocols is not intended to be all inclusive, but is focused on new tools and techniques—or new twists on old techniques— that will likely be widely used in the coming decade. There are several wellestablished E. coli expression systems (e.g., the original T7 RNA polymerase expression strains and vectors developed by William F. Studier and colleagues; the use of GST and polyhistidine fusion tags for protein purification) that have been extensively described in other methods volumes and peer-reviewed journal articles and are thus not included in this volume, with the exception of a few contributions in which certain of these systems have been adapted for novel applications or otherwise improved upon.
Peter E. Vaillancourt - ❰ له مجموعة من الإنجازات والمؤلفات أبرزها ❞ Methods in Molecular Biology Expression Protocols ❝ ❱
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وصف الكتاب : نبذه عن الكتاب:

The aim of E. coli Gene Expression Protocols is to familiarize and
instruct the reader with currently popular and newly emerging methodologies
that exploit the advantages of using E. coli as a host organism for expressing
recombinant proteins. The chapters generally fall within two categories:
(1) the use of E. coli vectors and strains for production of pure, functional
protein, and (2) the use of E. coli as host for the functional screening of large
collections of proteins or peptides. These methods and protocols should be of
use to researchers over a wide range of disciplines. Chapters that fall within
the latter category describe protocols that will be particularly relevant for
functional genomics studies.
The chapters of E. coli Gene Expression Protocols are written by experts
who have hands-on experience with the particular method. Each article is
written in sufficient detail so that researchers familiar with basic molecular
techniques and experienced with handling E. coli and its bacteriophages should
be able to carry out the procedures successfully. As in all volumes of the
Methods in Molecular Biology series, each chapter includes an extensive Notes
section, in which practical details peculiar to the particular method are
described.
E. coli Gene Expression Protocols is not intended to be all inclusive, but
is focused on new tools and techniques—or new twists on old techniques—
that will likely be widely used in the coming decade. There are several wellestablished E. coli expression systems (e.g., the original T7 RNA polymerase
expression strains and vectors developed by William F. Studier and colleagues;
the use of GST and polyhistidine fusion tags for protein purification) that
have been extensively described in other methods volumes and peer-reviewed
journal articles and are thus not included in this volume, with the exception of
a few contributions in which certain of these systems have been adapted for
novel applications or otherwise improved upon.

سنة النشر : 2003م / 1424هـ .
عدد مرات التحميل : 3630 مرّة / مرات.
تم اضافته في : الجمعة , 7 فبراير 2020م.
حجم الكتاب عند التحميل : 2.40 .

ولتسجيل ملاحظاتك ورأيك حول الكتاب يمكنك المشاركه في التعليقات من هنا:

Biologically

Biology is a natural science that is concerned with the study of life, its various forms and its function, how these organisms interact with each other and with the surrounding environment. The word biology in Greek is made up of two words: bio (βίος) meaning life. And loggia (-λογία) means science or study. Biology: the similarity of vegetation and animal cover on the edges of the African and American states, and the existence of the same fossil.


Branches of biology
Biology is an ancient science thousands of years old and modern biology began in the nineteenth century. This science has multiple branches. Among them are:

Anatomy
Botany
Biochemia
Biogeography
Biofisia
Cytology or cell science
Ecology or environmental science

 

 

نبذه عن الكتاب:

The aim of E. coli Gene Expression Protocols is to familiarize and
instruct the reader with currently popular and newly emerging methodologies
that exploit the advantages of using E. coli as a host organism for expressing
recombinant proteins. The chapters generally fall within two categories:
(1) the use of E. coli vectors and strains for production of pure, functional
protein, and (2) the use of E. coli as host for the functional screening of large
collections of proteins or peptides. These methods and protocols should be of
use to researchers over a wide range of disciplines. Chapters that fall within
the latter category describe protocols that will be particularly relevant for
functional genomics studies.
The chapters of E. coli Gene Expression Protocols are written by experts
who have hands-on experience with the particular method. Each article is
written in sufficient detail so that researchers familiar with basic molecular
techniques and experienced with handling E. coli and its bacteriophages should
be able to carry out the procedures successfully. As in all volumes of the
Methods in Molecular Biology series, each chapter includes an extensive Notes
section, in which practical details peculiar to the particular method are
described.
E. coli Gene Expression Protocols is not intended to be all inclusive, but
is focused on new tools and techniques—or new twists on old techniques—
that will likely be widely used in the coming decade. There are several wellestablished E. coli expression systems (e.g., the original T7 RNA polymerase
expression strains and vectors developed by William F. Studier and colleagues;
the use of GST and polyhistidine fusion tags for protein purification) that
have been extensively described in other methods volumes and peer-reviewed
journal articles and are thus not included in this volume, with the exception of
a few contributions in which certain of these systems have been adapted for
novel applications or otherwise improved upon.

Biology
Human biology
Who is the founder of biology?
The importance of biology
Areas of work in the field of biology
Theories of biology
Research on biology for the first grade of secondary school
Human biology

 



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Peter E. Vaillancourt
Peter E. Vaillancourt



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